s1 omicron Search Results


omicron ba  (Sino Biological)
96
Sino Biological omicron ba
Heatmap showing binding of mAbs from C68 and C20 (rows) to spike antigens by ELISAs. Spaces are colored based on the OD450nm values with higher numbers and more binding represented in darker green. Antigens (columns) included the following: SARS-CoV-2 WH-1 S2 subunit protein produced in mammalian cells (Mam) or insect cells (Ins), SARS-CoV-2 pre-fusion stabilized spike trimers from WH-1 and VOCs (Delta, Omicron VOCs BA.1, BA.2 <t>BA.5,</t> BQ.1.1, XBB), SARS-CoV-1, MERS-CoV, HCoV-HKU1, and MERS-CoV S2 monomer protein. All mAbs were tested at 1 μg/mL, except against the SARS-CoV-2 S2 protein the mAbs were tested at 1 μg/mL or 15 μg/mL as noted. Values were averaged over two technical replicates.
Omicron Ba, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
omicron ba - by Bioz Stars, 2026-02
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sars cov 2 spike s1  (Native Antigen Inc)
93
Native Antigen Inc sars cov 2 spike s1
Heatmap showing binding of mAbs from C68 and C20 (rows) to spike antigens by ELISAs. Spaces are colored based on the OD450nm values with higher numbers and more binding represented in darker green. Antigens (columns) included the following: SARS-CoV-2 WH-1 S2 subunit protein produced in mammalian cells (Mam) or insect cells (Ins), SARS-CoV-2 pre-fusion stabilized spike trimers from WH-1 and VOCs (Delta, Omicron VOCs BA.1, BA.2 <t>BA.5,</t> BQ.1.1, XBB), SARS-CoV-1, MERS-CoV, HCoV-HKU1, and MERS-CoV S2 monomer protein. All mAbs were tested at 1 μg/mL, except against the SARS-CoV-2 S2 protein the mAbs were tested at 1 μg/mL or 15 μg/mL as noted. Values were averaged over two technical replicates.
Sars Cov 2 Spike S1, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
sars cov 2 spike s1 - by Bioz Stars, 2026-02
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bv421 labeled omicron spike  (Sino Biological)
92
Sino Biological bv421 labeled omicron spike
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Bv421 Labeled Omicron Spike, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
bv421 labeled omicron spike - by Bioz Stars, 2026-02
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recombinant trimeric omicron ba 1  (BPS Bioscience)
94
BPS Bioscience recombinant trimeric omicron ba 1
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Recombinant Trimeric Omicron Ba 1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant trimeric omicron ba 1 - by Bioz Stars, 2026-02
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omicron ba 1 s1  (Sino Biological)
95
Sino Biological omicron ba 1 s1
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Omicron Ba 1 S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
omicron ba 1 s1 - by Bioz Stars, 2026-02
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eg 5 1  (Sino Biological)
94
Sino Biological eg 5 1
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Eg 5 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eg 5 1/product/Sino Biological
Average 94 stars, based on 1 article reviews
eg 5 1 - by Bioz Stars, 2026-02
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xbb 1 16  (Sino Biological)
94
Sino Biological xbb 1 16
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Xbb 1 16, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
xbb 1 16 - by Bioz Stars, 2026-02
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rec32007  (Native Antigen Inc)
89
Native Antigen Inc rec32007
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Rec32007, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rec32007/product/Native Antigen Inc
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sars cov 2 b q 1 1 trimer  (Sino Biological)
94
Sino Biological sars cov 2 b q 1 1 trimer
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Sars Cov 2 B Q 1 1 Trimer, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s ecd homotrimers  (Sino Biological)
94
Sino Biological s ecd homotrimers
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
S Ecd Homotrimers, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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recombinant a 1  (Sino Biological)
93
Sino Biological recombinant a 1
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Recombinant A 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant a 1 - by Bioz Stars, 2026-02
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jo urn al pr eroo f  (Sino Biological)
94
Sino Biological jo urn al pr eroo f
Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron <t>spike-BV421+.</t> B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.
Jo Urn Al Pr Eroo F, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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Image Search Results


Heatmap showing binding of mAbs from C68 and C20 (rows) to spike antigens by ELISAs. Spaces are colored based on the OD450nm values with higher numbers and more binding represented in darker green. Antigens (columns) included the following: SARS-CoV-2 WH-1 S2 subunit protein produced in mammalian cells (Mam) or insect cells (Ins), SARS-CoV-2 pre-fusion stabilized spike trimers from WH-1 and VOCs (Delta, Omicron VOCs BA.1, BA.2 BA.5, BQ.1.1, XBB), SARS-CoV-1, MERS-CoV, HCoV-HKU1, and MERS-CoV S2 monomer protein. All mAbs were tested at 1 μg/mL, except against the SARS-CoV-2 S2 protein the mAbs were tested at 1 μg/mL or 15 μg/mL as noted. Values were averaged over two technical replicates.

Journal: bioRxiv

Article Title: The S2 subunit of spike encodes diverse targets for functional antibody responses to SARS-CoV-2

doi: 10.1101/2024.02.26.582219

Figure Lengend Snippet: Heatmap showing binding of mAbs from C68 and C20 (rows) to spike antigens by ELISAs. Spaces are colored based on the OD450nm values with higher numbers and more binding represented in darker green. Antigens (columns) included the following: SARS-CoV-2 WH-1 S2 subunit protein produced in mammalian cells (Mam) or insect cells (Ins), SARS-CoV-2 pre-fusion stabilized spike trimers from WH-1 and VOCs (Delta, Omicron VOCs BA.1, BA.2 BA.5, BQ.1.1, XBB), SARS-CoV-1, MERS-CoV, HCoV-HKU1, and MERS-CoV S2 monomer protein. All mAbs were tested at 1 μg/mL, except against the SARS-CoV-2 S2 protein the mAbs were tested at 1 μg/mL or 15 μg/mL as noted. Values were averaged over two technical replicates.

Article Snippet: The recombinant spike trimers used in these assays were as follows: Wuhan-Hu-1 (Sino Biological, cat. 40589-V08H4), Delta (Sino Biological, cat. 40589-V08H10), Omicron BA.1 (Sino Biological, cat. 40589-V08H26), Omicron BA.2 (Sino Biological, cat. 40589-V08H28), Omicron BA.4/BA.5 (Sino Biological, cat. 40589-V08H32), Omicron XBB (Sino Biological, cat. 40589-V08H40), Omicron BQ.1.1 (Sino Biological, cat. 40589-V08H41), SARS-CoV-1 (Acro Biosystems, cat. SPN-S52Ht), MERS-CoV (Acro Biosystems, cat. SPN-M52H4), HCoV-HKU1 (Acro Biosystems, cat.SPN-H52H5).

Techniques: Binding Assay, Produced

(A) Screening of all S2 mAbs for neutralization of WH-1 spike-pseudotyped lentiviruses. All antibodies were tested at 10 μg/mL and the target cells were ACE2-expressing HEK293T cells. The percent neutralization of the pseudoviruses is shown, averaged across two technical replicates. One mAb, C20.119, was able to neutralize greater than 50%, which was the threshold for further assessment, shown by the dashed line. Neutralization of a panel of SARS-CoV-2 variant pseudoviruses (B) or sarbecovirus pseudoviruses (C) by C20.119. SARS-CoV-2 pseudoviruses tested included WH-1, Delta, Omicron VOCs BA.1, BA.2, BA.5, BQ.1.1, XBB, XBB.1.5. The related sarbecovirus pseudoviruses assessed were SARS-CoV-1, bat viruses RsSHC014 and WIV1, and Pangolin-GD. C20.119 was tested in 12-point dilution curves starting at 20 μg/mL for WH-1 and Delta and 80 μg/mL for the other pseudoviruses. Fraction infectivity values are the average of two technical replicates in two to three independent experiments using at least two pseudovirus batches. (D) Pseudovirus neutralization IC50 values for C20.119.

Journal: bioRxiv

Article Title: The S2 subunit of spike encodes diverse targets for functional antibody responses to SARS-CoV-2

doi: 10.1101/2024.02.26.582219

Figure Lengend Snippet: (A) Screening of all S2 mAbs for neutralization of WH-1 spike-pseudotyped lentiviruses. All antibodies were tested at 10 μg/mL and the target cells were ACE2-expressing HEK293T cells. The percent neutralization of the pseudoviruses is shown, averaged across two technical replicates. One mAb, C20.119, was able to neutralize greater than 50%, which was the threshold for further assessment, shown by the dashed line. Neutralization of a panel of SARS-CoV-2 variant pseudoviruses (B) or sarbecovirus pseudoviruses (C) by C20.119. SARS-CoV-2 pseudoviruses tested included WH-1, Delta, Omicron VOCs BA.1, BA.2, BA.5, BQ.1.1, XBB, XBB.1.5. The related sarbecovirus pseudoviruses assessed were SARS-CoV-1, bat viruses RsSHC014 and WIV1, and Pangolin-GD. C20.119 was tested in 12-point dilution curves starting at 20 μg/mL for WH-1 and Delta and 80 μg/mL for the other pseudoviruses. Fraction infectivity values are the average of two technical replicates in two to three independent experiments using at least two pseudovirus batches. (D) Pseudovirus neutralization IC50 values for C20.119.

Article Snippet: The recombinant spike trimers used in these assays were as follows: Wuhan-Hu-1 (Sino Biological, cat. 40589-V08H4), Delta (Sino Biological, cat. 40589-V08H10), Omicron BA.1 (Sino Biological, cat. 40589-V08H26), Omicron BA.2 (Sino Biological, cat. 40589-V08H28), Omicron BA.4/BA.5 (Sino Biological, cat. 40589-V08H32), Omicron XBB (Sino Biological, cat. 40589-V08H40), Omicron BQ.1.1 (Sino Biological, cat. 40589-V08H41), SARS-CoV-1 (Acro Biosystems, cat. SPN-S52Ht), MERS-CoV (Acro Biosystems, cat. SPN-M52H4), HCoV-HKU1 (Acro Biosystems, cat.SPN-H52H5).

Techniques: Neutralization, Expressing, Variant Assay, Infection

Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron spike-BV421+. B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.

Journal: medRxiv

Article Title: Systems biological assessment of the temporal dynamics of immunity to a viral infection in the first weeks and months of life

doi: 10.1101/2023.01.28.23285133

Figure Lengend Snippet: Memory B and T cell response, related to A) Gating strategy used for SARS-CoV-2 spike specific IgG+ memory B cell staining and single-cell sorting. Gating was on singlets that were CD20+ and CD3-CD14-IgM-IgD-CD27low/+ IgG+. Sorted cells were Wuhan spike-AlexaFluor 488+ and/or Omicron spike-BV421+. B) The percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in healthy, acute, and convalescent infant individuals. The sample number for each group is indicated in brackets. C) As in (B), the percentage of SARS-CoV-2 spike-specific IgG+ memory B cells in adult individuals with mild, severe, and ICU symptoms and in adult convalescent individuals. The sample number for each group is indicated in brackets. D-F) T cells were stimulated with overlapping peptides against WT (D-F) and Omicron (F) variants. Cytokine production was determined via flow cytometry. D) Box plot showing the fraction of responding T cells at different infection stages. E) Box plot showing the fraction of multifunctional T cells at different infection stages. F) Comparison of the multifunctional T cell response after stimulation with WT and Omicron (Om) peptides. Statistical comparisons were conducted with Wilcoxon rank sum test. Solid line indicates median healthy response; dashed line indicates 3x median healthy response.

Article Snippet: The following antibodies were used: IgD PE (BD Biosciences, 555779), IgM PerCP-Cy5.5 (BioLegend, 314512), CD20 APC-H7 (BD Biosciences, 560734), CD27 PE-Cy7 (BioLegend, 302838), CD14 PE/Dazzle™ 594 (BioLegend, 301852), CD16 BV605 (BioLegend, 302040), IgG BV650 (BD Biosciences, 740596), CD3 BUV737 (BD Biosciences, 612750) and Alexa Fluor 488-labeled Wuhan spike (SinoBiological, 40589-V27B-B), and BV421 labeled Omicron Spike (SinoBiological™, 40589-V49H3-B).

Techniques: Staining, FACS, Flow Cytometry, Infection